[DSMZ 공식대리점 관련 업무대행/수입] NCI-H929

어스바이오는 DSMZ 전문 취급점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
DSMZ의 Cell line을 소개드립니다.
 

 

Cell line: NCI-H929
DSMZ no.: ACC 163
Species: human (Homo sapiens)
Cell type: multiple myeloma
Biosafety level: 1

DSMZ Cell Culture Data:
 

Morphology: single, round to oval cells or clustered in small clumps in suspension
Medium: 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 50 µM mercaptoethanol
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 70-80 hours
Harvest: maximum density at 1.5*10⁶ cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR -, cyIgG -, cyIgM -, cykappa +, cylambda -
Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics: human hypodiploid karyotype with 16% polyploidy - 45(43-46)<2n>X, -X, +8, -10, -13, +mar, dup(1)(q11q25), del(1)(p11p25), del(6)(q25), der(8)t(8;?)(q23;?)x2, del(9)(p13), del(12)(p12)
 

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